A 6 month-old male is thought to have sickle cell anemia, and as his pediatrician you want to sequence the gene that may be mutated, causing this disease. You decide to sequence the gene for the beta subunit of hemoglobin. In order to do this you must perform polymerase chain reaction (PCR). You need to know which of the following in order to amplify (PCR) the area of interest of his DNA?
A. The predicted change in nucleotide sequence
B. The nucleotide sequence in the areas upstream and downstream to the nucleotides of interest
C. The predicted change in amino acid sequence of the mutated protein
D. The change in number of hydrogen bonds within the nucleotide sequence of interest
E. The predicted change in size of the mutated protein
A. The predicted change in nucleotide sequence
B. The nucleotide sequence in the areas upstream and downstream to the nucleotides of interest
C. The predicted change in amino acid sequence of the mutated protein
D. The change in number of hydrogen bonds within the nucleotide sequence of interest
E. The predicted change in size of the mutated protein